Integrated Genomics Shared Resource in the Georgia Cancer Center

The Integrated Genomics Shared Resource in the Georgia Cancer Center houses a complete Illumina NextGen Sequencing Facility including both HiSeq, MiSeq, and NextSeq 500 instruments.

Expertise for performing DNA-Seq, RNA-Seq, Chip-Seq, Methyl-Seq and targeted re-sequencing is available. Microarray resources offer the Affymetrix  Platform in order to facilitate exon-specific, oligonucleotide and genome-wide arrays.

The Resource also offers single cell sequencing analysis using the Chromium Controller System. Bioinformatics support is available for data analysis for both sequencing and microarray data.

Partek Genomics Suite and Ingenuity Pathways Analysis Softwares are available for analyses of genomic data.

EQUIPMENT / INFRASTRUCTURE SEQUENCING

• 10X Genomics Chromium Controller
• Agilent ® 2100 Bioanalyzer
• Agilent ® 2200 TapeStation
• NanoDrop 1000
• QuBit Fluorometer
• Illumina HiSeq 2500 (Rapid & Hi-Throughput Modes)
• Illumina Miseq
• Illumna NextSeq 500
• cBot Cluster Generation System
• Applied Biosystems GeneAmp 9700 Thermocycler
• Pyrosequencer (Qiagen PyroMark MD / PyroMark Q96 workstation)
• Covaris

APPLICATIONS

·       DNA Sequencing

·       Gene Regulation Analysis

·       Sequencing-Based Transcriptome Analysis

·       SNP Discovery and Structural Variation Analysis

·       Cytogenetic Analysis

·       DNA-Protein Interaction Analysis (ChIP-Seq)

·       Sequencing-Based Methylation Analysis

·       Chromium Single Cell Gene Expression

EQUIPMENT / INFRASTRUCTURE MICROARRAY

• Affymetrix ® GeneChip Scanner 3000 7G Plus
• Affymetrix ® Hybridization Oven 640
• Affymetrix ® Fluidics Station 450 (x2)

DATA ANALYSIS *

• Affymetrix ® Genotyping Console
• Affymetrix ® Expression Console
• Partek
• Ingenuity Pathway Analysis